The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was performed by French Anderson in a trial starting in September 1990.
Naked DNA approaches have also been explored, especially in the context of vaccine development.Generally, efforts focused on administering a gene that causes a needed protein to be expressed.Gene therapy is defined by the precision of the procedure and the intention of direct therapeutic effects.DNA must be administered, reach the damaged cells, enter the cell and either express or disrupt a protein. The initial approach incorporated DNA into an engineered virus to deliver the DNA into a chromosome.Short (20-25 nucleotides in length), labeled probes are exposed to the non-fragmented target DNA, hybridization occurs with high specificity due to the short length of the probes and even a single base change will hinder hybridization.
The target DNA is then washed and the labeled probes that didn't hybridize are removed.
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Molecular biology concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, and proteins and their biosynthesis, as well as the regulation of these interactions.
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The reaction is extremely powerful and under perfect conditions could amplify 1 DNA molecule to become 1.07 billion molecules in less than 2 hours.